Fig 1: Validation of bulk RNA sequencing by real-time PCR and immunoblotting assays(A) Comparison of up-regulated gene expressions in granulocytes by real-time PCR; (B) Comparison of down-regulated gene expressions in granulocytes by real-time PCR; (C) Validation of TMEM176A, AMT, STARD4 and Erk/MAPK signaling pathway by western blot assay. Each plot represents a dependent individual. KD: Kimura’s disease; HC: Healthy control. Data are represented as mean ± SEM. *: p< 0.05, **: p< 0.01.